골막기원세포에서 strontium에 의한 조골세포 표현형의 활성
Stimulation of osteoblastic phenotypes by strontium in periosteal-derived cells
김신원, 김욱규, 박봉욱, 하영술, 조희영, 김정환, 김덕룡, 주현호, 변준호,
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김신원 ( Kim Shin-Won ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
김욱규 ( Kim Uk-Kyu ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
박봉욱 ( Park Bong-Wook ) - 경상대학교 의학전문대학원 치과학교실 구강악안면외과
하영술 ( Hah Young-Sool ) - 경상대학교병원 임상의학연구소
조희영 ( Cho Hee-Young ) - 경상대학교병원 임상의학연구소
김정환 ( Kim Jung-Hwan ) - 경상대학교병원 임상의학연구소
김덕룡 ( Kim Deok-Ryong ) - 경상대학교 의학전문대학원 생화학교실
주현호 ( Joo Hyun-Ho ) - 경상대학교 의학전문대학원 구강악안면외과학교실
변준호 ( Byun June-Ho ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
KMID : 0360120100320030199
Abstract
This study investigated the effects of strontium on osteoblastic phenotypes of cultured human periostealderived cells. Periosteal tissues were harvested from mandible during surgical extraction of lower impacted third molar. Periosteal-derived cells were introduced into cell culture. After passage 3, the periostealderived cells were further cultured for 28 days in an osteogenic induction DMEM medium supplemented with fetal bovine serum, ascorbic acid 2-phosphate, dexamethasone and at a density of cells/well in a 6-well plate. In this culture medium, strontium at different concentrations (1, 5, 10, and 100 /mL) was added. The medium was changed every 3 days during the incubation period. We examined the cellular proliferation, histochemical detection and biochemical measurements of alkaline phosphatase (ALP), the RT-PCR analysis for ALP and osteocalcin, and von Kossa staining and calcium contents in the periostealderived cells. Cell proliferation was not associated with the addition of strontium in periosteal-derived cells. The ALP activity in the periosteal-derived cells was higher in 5, 10, and 100 /ml strontium-treated cells than in untreated cells at day 14 of culture. Among the strontium-treated cells, the ALP activity was appreciably higher in 100 /ml strontium-treated cells than in 5 and 10 /ml strontium-treated cells. The levels of ALP and osteocalcin mRNA in the periosteal-derived cells was also higher in strontium-treated cells than in untreated cells at day 14 of culture. Their levels were increased in a dose-dependent manner. Von Kossa-positive mineralization nodules were strongly observed in the 1 /ml strontium-treated cells at day 21 and 28 of culture. The calcium content in the periosteal-derived cells was also higher in 1 /ml strontium-treated cells at day 28 of culture. These results suggest that low concentration of strontium stimulates the osteoblastic phenotypes of more differentiated periosteal-derived cells, whereas high concentration of strontium stimulates the osteoblastic phenotypes of less differentiated periosteal-derived cells. The effects of strontium on osteoblastic phenotypes of periosteal-derived cells appear to be associated with differentiation-extent.
키워드
Periosteal-derived cells; Strontium; Osteoblastic phenotypes
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